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Team:Cambridge/Experiments
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| - | ===[[Team:Cambridge/Experiments/Squid_Dissection_and_Tissue_Sample | Amplification of reflectin genes from squid genomic DNA]]=== | + | ===[[Team:Cambridge/Experiments/Squid_Dissection_and_Tissue_Sample | Amplification of reflectin genes from the squid genomic DNA]]=== |
Reflectin genes were amplified directly from [http://en.wikipedia.org/wiki/Loligo ''Loligo''] tissue. Tissue from the ''Loligo'' genus was commercially available from fishing bait suppliers and culinary wholesalers. Primers were designed from the nucleotide sequences of three reflectin proteins identified in ''L. pealei'', and used in a [[Team:Cambridge/Protocols/PCR|PCR]] reaction upon ''L. vulgaris'' [[Team:Cambridge/Protocols/Extraction of genomic DNA from squid| genomic DNA.]] | Reflectin genes were amplified directly from [http://en.wikipedia.org/wiki/Loligo ''Loligo''] tissue. Tissue from the ''Loligo'' genus was commercially available from fishing bait suppliers and culinary wholesalers. Primers were designed from the nucleotide sequences of three reflectin proteins identified in ''L. pealei'', and used in a [[Team:Cambridge/Protocols/PCR|PCR]] reaction upon ''L. vulgaris'' [[Team:Cambridge/Protocols/Extraction of genomic DNA from squid| genomic DNA.]] | ||
{{Template:Team:Cambridge/CAM_2011_TEMPLATE_FOOT}} | {{Template:Team:Cambridge/CAM_2011_TEMPLATE_FOOT}} | ||
Revision as of 15:22, 1 August 2011
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Contents |
Experiments
Details of the experiments carried out throughout the project are linked from this page. These experiments should also be linked to from the appropriate diary page.
Preliminary Exercise
Initial exercise during our 2 weeks crash course in synthetic biology with the aim of familiarising us with common laboratory methods of preparing and assembling DNA.
Main Project - 'Bactiridescence'
Amplification of reflectin genes from the squid genomic DNA
Reflectin genes were amplified directly from [http://en.wikipedia.org/wiki/Loligo Loligo] tissue. Tissue from the Loligo genus was commercially available from fishing bait suppliers and culinary wholesalers. Primers were designed from the nucleotide sequences of three reflectin proteins identified in L. pealei, and used in a PCR reaction upon L. vulgaris genomic DNA.
