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Team:Cambridge/Protocols/Transformation of B. subtilis
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| - | == | + | ==Transformation of ''Bacillus subtilis''== |
| - | + | A technique used to introduce foreign DNA into competent Bacillus cells. | |
===Theory=== | ===Theory=== | ||
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===Practice=== | ===Practice=== | ||
Revision as of 10:46, 19 July 2011
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Transformation of Bacillus subtilis
A technique used to introduce foreign DNA into competent Bacillus cells.
Theory
Practice
- Add to a clean Eppendorf tube:
Reagent Quantity competent Bacillus cells 400μl DNA 3μl
- Incubate at 37ºC for 1 hour.
- Plate on an agar plate containing antibiotic, which allows for selection of successful transformants.
Safety
All equipment (including gloves) that may have come into contact with the bacteria must be autoclaved for decontamination purposes.
