"
Team:Cambridge/Experiments/Initial Exercise Group control
From 2011.igem.org
(Difference between revisions)
(→Positive Control Experiment) |
(→Positive Control Experiment) |
||
| Line 2: | Line 2: | ||
==Positive Control Experiment== | ==Positive Control Experiment== | ||
| + | |||
| + | ===Construct Design=== | ||
| + | In the positive control experiment we replaced the Green Fluorescent Protein coding sequence with a coding sequence for mRUBY, which is a Bright Monomeric Red Fluorescent Protein. | ||
| + | The picture below shows a map of the modified plasmid. | ||
The control experiment consists of 3 reactions | The control experiment consists of 3 reactions | ||
Revision as of 09:04, 15 July 2011
Loading...
Positive Control Experiment
Construct Design
In the positive control experiment we replaced the Green Fluorescent Protein coding sequence with a coding sequence for mRUBY, which is a Bright Monomeric Red Fluorescent Protein. The picture below shows a map of the modified plasmid.
The control experiment consists of 3 reactions
- Reaction A
- 1μl primer ruby F
- 1μl primer ruby R
- 1μl mRuby (Elliot)
- Reaction B
- 1μl primer Vector F
- 1μl primer B reverse (provided)
- 1μl vector template
- Reaction C
- 1μl primer Vector R
- 1μl primer A forward (provided)
- 1μl vector template
