Team:Cambridge/Experiments/Initial Exercise Group One

From 2011.igem.org

(Difference between revisions)
(→Initial Exercise: Katy, Heather, Marta and Veronica)
(→Initial Exercise: Katy, Heather, Marta and Veronica)
Line 13: Line 13:
*some small anucleate cells*
*some small anucleate cells*
-
===Reasons for choice===
+
===Reasons for Choice===
*constitutively expressed*
*constitutively expressed*
*Distinct characteristic distribution that is easy to observe*
*Distinct characteristic distribution that is easy to observe*
*successful divIVA-GFP fusion documented by Edwards and ERrington, 1997*
*successful divIVA-GFP fusion documented by Edwards and ERrington, 1997*
 +
 +
===Primer Design===
 +
We wish to:
 +
*keep the original promotor for the Div IVA*
 +
*remove GFP promotor sequence*
 +
*remove DivivA stop codon*
 +
*Remove ComGA stabiliser sequence*
 +
*‘Linkers’ were described by Edwards and Errington - we suspect that this is related to the restriction enzyme assembly process they used and hence didn’t include it in our design*
{{Template:Team:Cambridge/CAM_2011_TEMPLATE_FOOT}}
{{Template:Team:Cambridge/CAM_2011_TEMPLATE_FOOT}}

Revision as of 22:36, 6 July 2011

Loading...
OVERVIEW
home

Contents

Initial Exercise: Katy, Heather, Marta and Veronica

Fusion of Div IVA protein with GFP

Role of Div IVA

Div IVA controls septum positioning in Bacillus Subtilis

Mutants show

  • inhibition of cell division initiation*
  • abnormally placed septum*
  • some small anucleate cells*

Reasons for Choice

  • constitutively expressed*
  • Distinct characteristic distribution that is easy to observe*
  • successful divIVA-GFP fusion documented by Edwards and ERrington, 1997*

Primer Design

We wish to:

  • keep the original promotor for the Div IVA*
  • remove GFP promotor sequence*
  • remove DivivA stop codon*
  • Remove ComGA stabiliser sequence*
  • ‘Linkers’ were described by Edwards and Errington - we suspect that this is related to the restriction enzyme assembly process they used and hence didn’t include it in our design*


Retrieved from "http://2011.igem.org/Team:Cambridge/Experiments/Initial_Exercise_Group_One"