Team:Brown-Stanford/Lab/Protocols/CaCl

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<html><h1>Protocol Code: CT3</h1></html>
<html><h1>Protocol Code: CT3</h1></html>

Revision as of 17:39, 27 September 2011

Brown-Stanford
iGEM
Back to
iGEM HQ

Protocol Code: CT3

CaCl2 Competency

Buffers

TfBI

  • 30mM KOAc
  • 50mM MnCl2-4H2O
  • 100mM KCl
  • 10mM CaCl2-2H2O
  • 15% Glycerol

TfBII

  • 10mM Na-MOPS pH 7
  • 75mM CaCl2
  • 10mM KCl
  • 10% Glycerol

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