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Team:Cambridge/Experiments/Thin Films
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(Created page with "{{Template:Team:Cambridge/CAM_2011_TEMPLATE_HEAD}} We conducted a number of experiments with the thin films, outlined below. ====[[Team:Cambridge/Experiments/Reflectin_Thin_Fil...") |
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* Introducing the 'Piranha solution' for surface cleaning | * Introducing the 'Piranha solution' for surface cleaning | ||
| - | ====[[Team:Cambridge/Experiments/Reflectin_Thin_Films_V | Uniform Films]]==== | + | ====[[Team:Cambridge/Experiments/Reflectin_Thin_Films_V | Uniform Films and Multilayers]]==== |
* Quality over quantity - purer reflectin and greater control over spin lead to colour uniformity | * Quality over quantity - purer reflectin and greater control over spin lead to colour uniformity | ||
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* Attempts to create multilayers with alternating PDMS and reflectin. | * Attempts to create multilayers with alternating PDMS and reflectin. | ||
| - | * Problems with stability of reflectin due to impurities hinder progress | + | * Problems with stability of reflectin due to impurities hinder progress but produce 'protein art' |
| - | ====[[Team:Cambridge/Experiments/ | + | ====[[Team:Cambridge/Experiments/Reflectin_Thin_Films_VI | Attempts to increase film stability lead to first multilayer]]==== |
* Dilution and centrifuging of samples and using only the very top layer seem to provide better stability for urea containing reflectin | * Dilution and centrifuging of samples and using only the very top layer seem to provide better stability for urea containing reflectin | ||
* Dialysed protein seemed to be more stable however suffers from dewetting | * Dialysed protein seemed to be more stable however suffers from dewetting | ||
{{Template:Team:Cambridge/CAM_2011_TEMPLATE_FOOT}} | {{Template:Team:Cambridge/CAM_2011_TEMPLATE_FOOT}} | ||
Revision as of 11:12, 21 September 2011
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We conducted a number of experiments with the thin films, outlined below.
Contents |
Reflectin Thin Films I
- Description of the basic method
- Colourful thin films created with reflectin & HFIP
- Thin film colour not uniform
- HFIP thin film control did not show structural colour
Reflectin Thin Films II
- Method refined in an attempt to reduce impurities
- Bovine Serum Album (BVA) control to test whether generic proteinacious thin films produce colour
- Did not show structural colour
- Reflectin thin films appear to crystalize on drying
Reflectin Thin Films III
- Control indicates that Urea may be to blame for thin film crystalization
- More controls show that neither GFP nor BSA exhibit structural colour when spun into thin films
Colour Intense Films - First Breakthrough
- Less is more - using the same concentration, spinning a small volume leads to better wetting and more colour intense films
- Introducing the 'Piranha solution' for surface cleaning
Uniform Films and Multilayers
- Quality over quantity - purer reflectin and greater control over spin lead to colour uniformity
- Attempts to create multilayers with alternating PDMS and reflectin.
- Problems with stability of reflectin due to impurities hinder progress but produce 'protein art'
Attempts to increase film stability lead to first multilayer
- Dilution and centrifuging of samples and using only the very top layer seem to provide better stability for urea containing reflectin
- Dialysed protein seemed to be more stable however suffers from dewetting
