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Team:Amsterdam/5 September 2011
From 2011.igem.org
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==Growth curves== | ==Growth curves== | ||
Still measuring the growth curves for 4 and 8°C | Still measuring the growth curves for 4 and 8°C | ||
| + | |||
| + | ==Constructs== | ||
| + | Constructs making using both the 2a and 3a assembly method. | ||
| + | Digestion of different parts to construct the following assemblies: | ||
| + | *R0010+ B0034 + CspC | ||
| + | *R0010+ B0034 + Cpn10 | ||
| + | *R0010+ B0034 + Cpn60 | ||
| + | *R0010+ B0034 + Shednak | ||
| + | *K206000+ B0034 + CspC | ||
| + | *K206000+ B0034 + Cpn10 | ||
| + | *K206000+ B0034 + Cpn60 | ||
| + | *K206000+ B0034 + Shednak <br> | ||
| + | These assemblies were ligated ON at 16°C. | ||
| + | |||
| + | ==Backbone transferring== | ||
| + | Digestion of different parts to tranfer to the Chloramphenicol backbone: | ||
| + | *All genes | ||
| + | *The constructs we got thus far | ||
| + | These parts were ligated into the Chloramphenicol backbone ON at 16°C. | ||
{{:Team:Amsterdam/Footer}} | {{:Team:Amsterdam/Footer}} | ||
Revision as of 07:59, 21 September 2011
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5-09-2011
Growth curves
Still measuring the growth curves for 4 and 8°C
Constructs
Constructs making using both the 2a and 3a assembly method. Digestion of different parts to construct the following assemblies:
- R0010+ B0034 + CspC
- R0010+ B0034 + Cpn10
- R0010+ B0034 + Cpn60
- R0010+ B0034 + Shednak
- K206000+ B0034 + CspC
- K206000+ B0034 + Cpn10
- K206000+ B0034 + Cpn60
- K206000+ B0034 + Shednak
These assemblies were ligated ON at 16°C.
Backbone transferring
Digestion of different parts to tranfer to the Chloramphenicol backbone:
- All genes
- The constructs we got thus far
These parts were ligated into the Chloramphenicol backbone ON at 16°C.
