Team:Amsterdam/5 September 2011

From 2011.igem.org

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05-09-2011

Growth curves

Still measuring the growth curves for 4 and 8°C

Constructs

Constructs making using both the 2a and 3a assembly method. Digestion of different parts to construct the following assemblies:

  • R0010+ B0034 + CspC
  • R0010+ B0034 + Cpn10
  • R0010+ B0034 + Cpn60
  • R0010+ B0034 + Shednak
  • K206000+ B0034 + CspC
  • K206000+ B0034 + Cpn10
  • K206000+ B0034 + Cpn60
  • K206000+ B0034 + Shednak

These assemblies were ligated ON at 16°C.

Backbone transferring

Digestion of different parts to tranfer to the Chloramphenicol backbone:

  • All genes
  • The constructs we got thus far

These parts were ligated into the Chloramphenicol backbone ON at 16°C.