"
Team:Cambridge/Protocols/Glycerol Stocks
From 2011.igem.org
(Difference between revisions)
(Created page with "{{Template:Team:Cambridge/CAM_2011_TEMPLATE_HEAD}} __NOTOC__ ==Glycerol Stocks== protocol description ===Theory=== How it works ===Practice=== How to do it in the lab {Standar...") |
(→Glycerol Stocks) |
||
| Line 4: | Line 4: | ||
==Glycerol Stocks== | ==Glycerol Stocks== | ||
protocol description | protocol description | ||
| + | |||
| + | 1. Peparation of liqid cultures - 100-fold dilution of overnight culture with selection. | ||
| + | 2. Incubation at 37° for 3-4 hours until mid-log phase. | ||
| + | 3. Mix with 60% glycerol to get a final concentration of 20%. | ||
| + | 4. Store at -80° | ||
===Theory=== | ===Theory=== | ||
Revision as of 17:07, 20 September 2011
Loading...
Glycerol Stocks
protocol description
1. Peparation of liqid cultures - 100-fold dilution of overnight culture with selection. 2. Incubation at 37° for 3-4 hours until mid-log phase. 3. Mix with 60% glycerol to get a final concentration of 20%. 4. Store at -80°
Theory
How it works
Practice
How to do it in the lab
{Standard layout for procedures is to use:
- <Procedure title - aka what you are doing>
- <step 1>
- <step 2>
- <additional notes/important information regarding the previous step>
the text within the < > is what should be written, don't include < > in actual writeup :P
if in doubt see the gel electrophoresis protocol
}
Safety
The safety implication of the procedure.
