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Team:Cambridge/Experiments/Periplasmic Export
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Revision as of 13:45, 20 September 2011
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Periplasmic export
As reflectins appear to spontaneously associate with membranes, we hoped to promote correct assembly by directing our recombinant reflectins to the [http://en.wikipedia.org/wiki/Periplasmic_space periplasmic space of E. coli].
Design of constructs
Choice of signal sequences
The signal sequence from TorA [http://www.ncbi.nlm.nih.gov/pubmed/11123687 has been used to export GFP to the periplasm]. Although
GFP imaging
Conclusion
