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Team:Cambridge
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| - | <p>We used Gibson Assembly | + | <p>We used Gibson Assembly exclusively for construct assembly during the competition, allowing us to assemble plasmids faster and with greater ease than would be possible using standard techniques.</p> |
<p>We contributed to a collection of software tools called <i>Gibthon</i> (initiated by Cambridge iGEM 2010), which aids the design of primers for Gibson assembly.</p> | <p>We contributed to a collection of software tools called <i>Gibthon</i> (initiated by Cambridge iGEM 2010), which aids the design of primers for Gibson assembly.</p> | ||
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Revision as of 17:19, 19 September 2011
Abstract
We investigated the properties of a novel yet under-researched group of proteins called reflectins.
Reflectins are interesting because of their self-organising properties, which cause them to produce dynamic structural colour.
We expressed codon-optimised reflectin in E.Coli, and observed the protein's in vivo and in vitro effects.
Structural Colour
Nature’s colours don’t just come from pigments, but from structure too.
Cephalopods camouflage themselves using intracellular structures made from reflectins.
These are the only known proteinaceous structures to exhibit structural colour. They are inspiring a new class of responsive optical materials.
Impact of iGEM
We wanted to know whether the iGEM has had any effect on its previous contestants.
To this end, we conducted a number of interviews with previous iGEMmers, as well as conducting a simple poll of past contestants.
Tools
We used Gibson Assembly exclusively for construct assembly during the competition, allowing us to assemble plasmids faster and with greater ease than would be possible using standard techniques.
We contributed to a collection of software tools called Gibthon (initiated by Cambridge iGEM 2010), which aids the design of primers for Gibson assembly.
