Team:Cambridge/Labwork/Protocols
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*[[Team:Cambridge/Protocols/Inclusion_Body_Prep | Inclusion Body Prep]] : A method to solubilise recombinant reflectin when it has been expressed in inclusion bodies in E. coli. | *[[Team:Cambridge/Protocols/Inclusion_Body_Prep | Inclusion Body Prep]] : A method to solubilise recombinant reflectin when it has been expressed in inclusion bodies in E. coli. | ||
*[[Team:Cambridge/Protocols/Protein_Purification | His-Trap Protein Purification]] : A method to purify reflectin from E. coli lysate using a his-trap column. | *[[Team:Cambridge/Protocols/Protein_Purification | His-Trap Protein Purification]] : A method to purify reflectin from E. coli lysate using a his-trap column. | ||
+ | |||
+ | '''Protein Purification''' | ||
+ | *[[Team:Cambridge/Protocols/Acetone_Precipitation_of_Proteins | Acetone Precipitation of proteins]] : A method to concentrate solutions of protein. | ||
+ | *[[Team:Cambridge/Protocols/Ethanol Precipitation_of_Proteins| Ethanol Precipitation of proteins]] : A method to concentrate solutions of protein. | ||
'''Thin Film Preparation''' | '''Thin Film Preparation''' |
Revision as of 18:45, 1 September 2011
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Protocols
A list of all protocols developed during the project. To add one, use the section below.
Amplification of DNA
- Polymerase Chain Reaction : A method for amplifying a section of DNA
- Colony PCR : A method allowing to check the length of an insert in an introduced plasmid
Analysis of DNA
- Gel Electrophoresis : A method used to separate DNA fragments of different sizes
- Gel Extraction of DNA : A technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis
- Rescue Precipitation of DNA : Creating a clean DNA solution after dissolving agarose gel.
- Restriction Enzyme Digestion : A method for creating a restriction map of a plasmid
Preparation of DNA Constructs
- Primer Design : Some general guidelines on how to design successful primers.
- Gibson Assembly : A relatively new technique of joining multiple DNA strands reliably
Transformation of Bacterial Cells
- Making Competent Cells : The methods required to make various cells competent
- Transformation of E.Coli : A simple method of transforming competent E.coli with your DNA of choice
- Tranformation of B. subtilis : A technique used to introduce foreign DNA into Bacillus cells.
- Transformation of E.coli by Electroporation: A technique for rapidly inserting DNA into cells, typically plasmid DNA, providing the cells are made competent in the correct manner.
Bacterial Cultures
- E. coli cell culture : A method for growing a cell culture in liquid medium
- Glycerol Stocks: A method of storing E.coli cells preserving their viability
Extraction of DNA from Cells
- Mini Prep : Extracting DNA from Bacterial Cells
- Extraction of genomic DNA from squid : A method to extract genomic DNA from squid tissue
- Extraction of cleaner genomic DNA from squid : A method to extract genomic DNA from squid tissue
Microscopy
- Confocal Microscopy : A method to visualise reflectins from squid samples.
- Slide Preparation for Confocal Microscopy : A method to perpare slides with agarose supplement to form microcolonies.
Protein Extraction
- Buffer Preparation : Methods to prepare the various buffers used to purify his-tagged reflectin from inclusion bodies in E. coli.
- Inclusion Body Prep : A method to solubilise recombinant reflectin when it has been expressed in inclusion bodies in E. coli.
- His-Trap Protein Purification : A method to purify reflectin from E. coli lysate using a his-trap column.
Protein Purification
- Acetone Precipitation of proteins : A method to concentrate solutions of protein.
- Ethanol Precipitation of proteins : A method to concentrate solutions of protein.
Thin Film Preparation
- Substrate Preparation:How to prepare a substrate for flow coating and spin coating.
- Spin Coating to Make a Thin Film: Our Spin Coating Protocol
- Flow Coating: How to flow coat a thin film.
Gel Electrophoresis by SDS PAGE
Adding new Protocols
To add a new protocol, enter the name in the box below and click new to create the new page. You must then return to this page in order to add in a link to the page by copying the code below.
Add a new link on this page with
#[[Team:Cambridge/Protocols/PROTOCOL_NAME_HERE |PROTOCOL NAME HERE]] : Insert description here