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Team:Cambridge/Experiments/Assembly of Reflectin Constructs
From 2011.igem.org
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==Construct Design== | ==Construct Design== | ||
==Primer Design== | ==Primer Design== | ||
| + | We should mention expected lengths of products here. | ||
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==Assembly: first attempt== | ==Assembly: first attempt== | ||
===PCR=== | ===PCR=== | ||
Revision as of 11:26, 19 August 2011
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Contents |
Construct Design
Primer Design
We should mention expected lengths of products here.
Assembly: first attempt
PCR
In the first round of PCR, we amplified fragments required for the assembly of GA1, GA2, GA3 and GA4 constructs.
- We performed PCR using Phusion Hot Start DNA Polymerase in 20 μl reaction volume.
Gibson Assembly
Transformation
Results
Diagnostics
Assembly: second attempt
PCR
Gibson Assembly
Transformation
Results
What next?
