Bactiridescence is a project based around the unique properties of reflectin, a squid protein with the highest refractive index of any known proteinaceous substance. In squid this protein forms complex platelets which act as [http://en.wikipedia.org/wiki/Distributed_Bragg_reflector Bragg reflectors] to provide camouflage. We aim to express reflectin in E. coli and optimise the optical properties in order to build the groundwork for the manipulation of living structural colour. Read more about reflectin and structural colour here.

Contents 1 Preliminary observations 2 Objective One - Express reflectin in E. coli 2.1 Isolating the reflectin gene 2.2 Selecting the promotor 2.3 Selecting the backbone 2.4 Creating the expression plasmid 2.5 Expression in E. coli 2.6 References

Preliminary observations

In order to get a real sense of what we were looking to achieve in our project, we felt that it was important to make some observations of native squid reflectin in vivo. We therefore obtained several specimens of loligo opalescens and loligo vulgaris squid from a local seafood restaurant and an online fishing bait store for dissection. We chose these species because the whole family of loliginid squid has been identified to contain reflectin, and these particular species were the only members of the family available to us. We used a confocal microscope to observe iridescent behaviour in eye and mantle tissue.

Objective One - Express reflectin in E. coli

Our first objective was to try to express reflectin, in any shape or form, in E. coli. Ideally, we'd be able to express reflectin in the bacteria at a range of levels using a single construct, so we can both overexpress (for in vitro studies) or underexpress (to promote in vivo folding) straightforwardly. In order to do this, we had to create an expression plasmid for reflectin with which we could transform the bacterial cells. Hence, we had to isolate a reflectin gene sequence, choose a suitable promotor and join them on an appropriate backbone in order to engineer what we wanted. These steps are outlined below.

Isolating the reflectin gene

This was

Selecting the promotor

Selecting the backbone

Creating the expression plasmid

Hopefully coming soon!

Expression in E. coli

Hopefully coming soon!

References

[http://www.nature.com/nmat/journal/v6/n7/abs/nmat1930.html] Kramer et al. The self-organizing properties of squid reflectin protein Nature Materials 533-538 VOL6 JULY 2007 [http://www.sciencemag.org/content/303/5655/235.short] Crookes et al. Reflectins: The Unusual Proteins of Squid Reflective Tissues SCIENCE 235-238 VOL303 9 JANUARY 2004 [http://www.sciencedirect.com/science/article/pii/S0142961209011442] Morse et al. The role of protein assembly in dynamically tunable bio-optical tissues Biomaterials 793-801 VOL31 FEBRUARY 2010 [http://www.publish.csiro.au/paper/ZO9920319.html] Griffiths et al. Iridophores in the mantle of giant clams. Australian Journal of Zoology (1992) Volume: 40, Issue: 3 Pages: 319-326 [http://www.ncbi.nlm.nih.gov/pubmed/19776150] Izumi et al. Changes in reflectin protein phosphorylation are associated with dynamic iridescence in squid. J. R. Soc. Interface 6 March 2010 vol. 7 no. 44 549-560 [http://www.springerlink.com/content/bba14b73ad35f495/]Brocco et al. Reflector cells in the skin of Octopus dofleini Cell and Tissue Research, Volume 205, Number 2, 167-186, 1980