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Team:Cambridge
From 2011.igem.org
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[[File:CAM_Iridescent.JPG | 400px | thumb | right | Iridescence in the eye of squid ''Loligo vulgaris'' ]] | [[File:CAM_Iridescent.JPG | 400px | thumb | right | Iridescence in the eye of squid ''Loligo vulgaris'' ]] | ||
| - | + | We have two principle branches to our main project; ''in vivo'' studies, in which we will be investigating the properties of reflectin protein in live bacteria, and ''in vitro'' studies, in which we will be investigating the properties of reflectin protein that has been extracted from E. coli. | |
| + | ''In vivo'', we believe that we will be able to engineer the reflectin gene such that it can be expressed in E. coli in a ‘properly folded’ state, something that has never been achieved before. We will then attempt to bind the protein to form an iridescent coating over the surface of the bacteria, creating what we term ‘bactiridescence’. If we could then control the colour changing behaviour of the reflectin, this could be a game-changing step for the future of biosensors. | ||
| - | + | ''In vitro'', we are confident that we can use the protein to make thin films with interesting optical behaviour (including colour change when someone breathes on it!) as well as diffraction gratings, which are virtually defect-free. There has already been an account of similar experiments in previous research, but we will be looking to extend it substantially. For example, we would like to create multi-layered thin films, which more accurately imitate the ‘natural’ arrangement of reflectin in squid tissue, and chemically induce colour changes, in ways that haven’t previously been attempted. These advances are necessary if reflectin is ever to be used in a commercial application. | |
| + | In addition, we are keen to expand the field of synthetic biology by making: | ||
| + | *Improved software tools. Our new open-source web-based software tool, PyGen, will be used for sequence manipulation and display, also integrating related applications already available on the web (including Gibthon, created by Cambridge iGEM 2010). It is specifically designed to improve on areas in which current researchers find fault with current software; we are conducting extensive research among the international iGEM community to ensure that it is consumer oriented. The open-source philosophy is deeply ingrained in the spirit of iGEM as well. | ||
| - | + | *Open-source enzymes. Currently, many universities struggle to find the funding necessary to participate in the iGEM competition, or synthetic biology in general. Commercial enzymes are often particularly expensive, placing a severe limitation on research. We aim to create a number of ‘BioBrick’ genes which will allow laboratories to ‘grow their own’; this should prove much cheaper than commercial sources. | |
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==Editing the wiki== | ==Editing the wiki== | ||
Revision as of 18:55, 2 August 2011
Welcome to the Cambridge iGEM 2011 team's wiki!
If you would like to contact the Cambridge iGEM 2011 team, please email us at cambridgeigem2011@gmail.com or find us on [http://twitter.com/#!/Cam_iGEM_2011 Twitter].
Bactiridescence
We have two principle branches to our main project; in vivo studies, in which we will be investigating the properties of reflectin protein in live bacteria, and in vitro studies, in which we will be investigating the properties of reflectin protein that has been extracted from E. coli.
In vivo, we believe that we will be able to engineer the reflectin gene such that it can be expressed in E. coli in a ‘properly folded’ state, something that has never been achieved before. We will then attempt to bind the protein to form an iridescent coating over the surface of the bacteria, creating what we term ‘bactiridescence’. If we could then control the colour changing behaviour of the reflectin, this could be a game-changing step for the future of biosensors.
In vitro, we are confident that we can use the protein to make thin films with interesting optical behaviour (including colour change when someone breathes on it!) as well as diffraction gratings, which are virtually defect-free. There has already been an account of similar experiments in previous research, but we will be looking to extend it substantially. For example, we would like to create multi-layered thin films, which more accurately imitate the ‘natural’ arrangement of reflectin in squid tissue, and chemically induce colour changes, in ways that haven’t previously been attempted. These advances are necessary if reflectin is ever to be used in a commercial application.
In addition, we are keen to expand the field of synthetic biology by making:
- Improved software tools. Our new open-source web-based software tool, PyGen, will be used for sequence manipulation and display, also integrating related applications already available on the web (including Gibthon, created by Cambridge iGEM 2010). It is specifically designed to improve on areas in which current researchers find fault with current software; we are conducting extensive research among the international iGEM community to ensure that it is consumer oriented. The open-source philosophy is deeply ingrained in the spirit of iGEM as well.
- Open-source enzymes. Currently, many universities struggle to find the funding necessary to participate in the iGEM competition, or synthetic biology in general. Commercial enzymes are often particularly expensive, placing a severe limitation on research. We aim to create a number of ‘BioBrick’ genes which will allow laboratories to ‘grow their own’; this should prove much cheaper than commercial sources.
Editing the wiki
To make a new page with all the right formatting, navigate to the page you want to create, click edit (you have to be logged in) and paste the following into the edit box, then work away. (I find [http://www.mediawiki.org/wiki/Help:Contents this] helpful.)
{{Template:Team:Cambridge/CAM_2011_TEMPLATE_HEAD}}
==Page Title==
Your text here
{{Template:Team:Cambridge/CAM_2011_TEMPLATE_FOOT}}
