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Team:Cambridge/Experiments/Isolating Reflectin DNA
From 2011.igem.org
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Obtaining DNA coding for reflectin proteins was the vital first step in our project. [http://en.wikipedia.org/wiki/Loligo Loligo] tissue was sourced from fishing bait suppliers and culinary wholesalers in order to attempt genomic DNA extraction. From our literature search we discovered that the reflectin gene from E. scolopes contains no introns, so genomic DNA should be suitable for expression in E. coli. | Obtaining DNA coding for reflectin proteins was the vital first step in our project. [http://en.wikipedia.org/wiki/Loligo Loligo] tissue was sourced from fishing bait suppliers and culinary wholesalers in order to attempt genomic DNA extraction. From our literature search we discovered that the reflectin gene from E. scolopes contains no introns, so genomic DNA should be suitable for expression in E. coli. | ||
==Squid Dissection and Tissue Samples== | ==Squid Dissection and Tissue Samples== | ||
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| + | Specimens of what we identified as [http://www.marlin.ac.uk/speciesinformation.php?speciesID=3718 ''Loligo vulgaris''] and [http://en.wikipedia.org/wiki/Opalescent_Inshore_Squid Loligo opalescens] were dissected for examination. | ||
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Revision as of 11:17, 20 July 2011
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Obtaining DNA coding for reflectin proteins was the vital first step in our project. [http://en.wikipedia.org/wiki/Loligo Loligo] tissue was sourced from fishing bait suppliers and culinary wholesalers in order to attempt genomic DNA extraction. From our literature search we discovered that the reflectin gene from E. scolopes contains no introns, so genomic DNA should be suitable for expression in E. coli.
Squid Dissection and Tissue Samples
Specimens of what we identified as [http://www.marlin.ac.uk/speciesinformation.php?speciesID=3718 Loligo vulgaris] and [http://en.wikipedia.org/wiki/Opalescent_Inshore_Squid Loligo opalescens] were dissected for examination.
