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Team:Cambridge/Protocols/Restriction Enzyme Digestion
From 2011.igem.org
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===Practice=== | ===Practice=== | ||
| - | Master mix preparation | + | * '''Master mix preparation''' |
| - | : | + | : 2.0μl of DNA |
| + | : 0.5μl of each restriction enzyme (or a respective amount of water for the control with uncut plasmids) | ||
| + | : 0.1μl of BSA - acetylated Bovine Serum Albumin enhances the performance of restriction enzymes | ||
| + | : 5.9μl of water | ||
| + | : 1.0μl of 2×NEBuffer | ||
===Safety=== | ===Safety=== | ||
Revision as of 13:47, 15 July 2011
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Restriction Enzyme Digestion
protocol description
Theory
How it works
Practice
- Master mix preparation
- 2.0μl of DNA
- 0.5μl of each restriction enzyme (or a respective amount of water for the control with uncut plasmids)
- 0.1μl of BSA - acetylated Bovine Serum Albumin enhances the performance of restriction enzymes
- 5.9μl of water
- 1.0μl of 2×NEBuffer
Safety
The safety implication of the procedure.
