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Team:Cambridge/Experiments/Initial Exercise Group control
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In the positive control experiment we replaced the Green Fluorescent Protein coding sequence with a coding sequence for mRUBY, which is a Bright Monomeric Red Fluorescent Protein. | In the positive control experiment we replaced the Green Fluorescent Protein coding sequence with a coding sequence for mRUBY, which is a Bright Monomeric Red Fluorescent Protein. | ||
The picture below shows a map of the modified plasmid. | The picture below shows a map of the modified plasmid. | ||
| + | [[File:cam_plasmid_positivecontrol.jpg | middle | thumb | 400px | map of the modified plasmid with mRUBY insertion]] | ||
The control experiment consists of 3 reactions | The control experiment consists of 3 reactions | ||
Revision as of 09:46, 15 July 2011
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Positive Control Experiment
Construct Design
In the positive control experiment we replaced the Green Fluorescent Protein coding sequence with a coding sequence for mRUBY, which is a Bright Monomeric Red Fluorescent Protein. The picture below shows a map of the modified plasmid.
The control experiment consists of 3 reactions
- Reaction A
- 1μl primer ruby F
- 1μl primer ruby R
- 1μl mRuby (Elliot)
- Reaction B
- 1μl primer Vector F
- 1μl primer B reverse (provided)
- 1μl vector template
- Reaction C
- 1μl primer Vector R
- 1μl primer A forward (provided)
- 1μl vector template
