Team:Brown-Stanford/Lab/Protocols
From 2011.igem.org
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=== '''Competency and Transformations''' === | === '''Competency and Transformations''' === | ||
+ | *[http://partsregistry.org/Help:Transformation_Protocol Registry Distribution Transformation] | ||
+ | *[https://2008.igem.org/Team:Hawaii/Protocols/Competent_Cells TOP10 Competency Protocol] | ||
*[[Team:Brown-Stanford/Lab/Protocols/FastCaCl|Fast CaCl<sub>2</sub> Combined Competency and Transformation]] | *[[Team:Brown-Stanford/Lab/Protocols/FastCaCl|Fast CaCl<sub>2</sub> Combined Competency and Transformation]] | ||
Revision as of 19:27, 30 June 2011
Protocols
Media
- BG-11
- LB (Lysogeny broth)
- ATCC medium: 1376 (Bacillus pasteurii NH4-YE medium)
- Enrichment media
- Nutrient agar with urea
- SOB (Super Optimal Broth) and SOC (Super Optimal broth with Catabolite repression)
Competency and Transformations
- [http://partsregistry.org/Help:Transformation_Protocol Registry Distribution Transformation]
- TOP10 Competency Protocol
- Fast CaCl2 Combined Competency and Transformation
Equipment