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Team:Cambridge/Parts
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==System diagrams== | ==System diagrams== | ||
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| + | Our parts were designed for the two main branches of our labwork - [[Team:Cambridge/Project/In_Vivo | ''in vivo'' expression and export]] to try and achieve structural colour, and [[Team:Cambridge/Project/In_Vitro | overexpression for purification]] and ''in vitro'' studies of our recombinant reflectins. | ||
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===Overexpression for purification=== | ===Overexpression for purification=== | ||
[[File:Cam_Overexpression_Construct.png | 600px]] | [[File:Cam_Overexpression_Construct.png | 600px]] | ||
Revision as of 22:54, 20 September 2011
System diagrams
Our parts were designed for the two main branches of our labwork - in vivo expression and export to try and achieve structural colour, and overexpression for purification and in vitro studies of our recombinant reflectins.
Overexpression for purification
Export
Featured Parts
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K638001 Reflectin A1]
Reflectins are a family of proteins which help give cephalopods their amazing camouflage abilities and which can self-assemble into a variety of multilayered structures with optical properties. We worked with Reflectin A1 from Loligo pealeii. See our background page for more information about reflectins and their role in cephalopod camouflage, and see [http://partsregistry.org/wiki/index.php?title=Part:BBa_K638001 our registry page] for information about our BioBrick submission.
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K638201 Reflectin A1 with poly-His tag]
In pure form reflectins may be used to create vibrantly coloured thin films and other devices. We used a poly-His affinity column to purify reflectin from cells for this purpose.
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K638402 Improved TorA tag]
We submitted a variant of the TorA leader sequence export tag. This TorA leader sequence variant has had been successfully used to export GFP to the periplasm of E.coli as described [http://www.ncbi.nlm.nih.gov/pubmed/11123687 here]. Our tag differs slightly from [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233307 the TorA tag already in the registry] and should be cheaper to obtain from primer synthesis.
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K638401 Reflectin A1 with N-terminal TorA tag]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K638403 Reflectin A1 with N-terminal TorA tag and C-terminal sfGFP]
...and the rest
A complete list of parts submitted to the Registry by this year's Cambridge team:
<groupparts>iGEM011 Cambridge</groupparts>
