Team:Cambridge

From 2011.igem.org

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Welcome to the Cambridge iGEM 2011 team's wiki!
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If you would like to contact the Cambridge iGEM 2011 team, please send us [mailto:cambridgeigem2011@gmail.com an email] or find us on [http://twitter.com/#!/Cam_iGEM_2011 Twitter].
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''Project outline here. Tell us more about your project. Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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==Bacteriadescence - Reflectin' the Future==
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Reflectins are a recently identified protein family rich in aromatic and sulphur-containing amino acids, responsible for the 'reflective' camouflage exhibited by certain cephalopods. To date, researchers have isolated the protein, reproduced it using ''Escherichia coli'' and shown it to exhibit self-assembling behaviour which lead to dynamic manipulation of incident light.
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        <p>Abstract</p>
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      <p>We investigated the properties of a novel yet under-researched group of proteins called reflectins.</p>
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      <p>Reflectins are interesting and unusual because of their self-organising properties, which cause them to produce dynamic structural colour.</p>
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      <p>We expressed codon-optimised reflectin in E. coli, and investigated the proteins' in vivo and in vitro effects.</p>
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Under in-vitro conditions ''Kramer et. al'' produced thin films, photonic gratings and fibres which exhibited  structural colour which extended across the visual spectrum by varying the thickness. In particular the colour change was demonstrated to be reversible. It is hypothesized the colouration is a result of thin film interference.
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        <p>Structural Colour</p>
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      <p>Nature’s colours don’t just come from pigments, but from structure too.</p>
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      <p>Cephalopods camouflage themselves using intracellular structures made from reflectins.</p>
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      <p>These are the only known proteinaceous structures to exhibit structural colour. They are inspiring a new class of responsive optical materials.</p>
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Within the squid ''Loligo pealeii'', ''Morse et.al'' found a multi-layer alternating structure of iridophore platelets containing spherical reflectin and extracellular space with different refractive indices. By studying tissue samples in-vitro the researchers observed conformation changes in the multi-layer structure due to phosphorylation allowed dynamic control of colour.
 
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As part of our iGEM project we propose to express reflectin in-vivo within ''Escherichia coli'' to reproduce the same multi-layer structure. Further we wish to demonstrate the ability to tune structural colour in-vivo through phosphorylation. Our work will directly impact upon the design of next-generation of novel biosensors.
 
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==WIKI NEWS==
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        <p>Impact of iGEM</p>
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        <p>We wanted to know what effect the iGEM competition has had on its past contestants.</p>
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        <p>We were also interested in what past participants thought of iGEM and how it shaped their view of synthetic biology.</p>
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        <p>We got in touch with a number of iGEM alumni and asked them to share their experience.</p>
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        <p>Tools</p>
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        <p>We used Gibson Assembly exclusively to build our constructs during the competition. This allowed us to do multi-part assemblies faster and with fewer bugs than if we had used standard techniques.</p>
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        <p>We contributed to a collection of software tools called <i>Gibthon</i> (initiated by Cambridge iGEM 2010), which aids the design of primers for Gibson assembly.</p>
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Check the safety page!
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Now we have some protocols also.
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==Editing the wiki==
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Please feel free to edit the wiki. It's pretty straightforward really - it's a glorified text editor! Don't worry about wanting to make stuff private or invisible, this doesn't have to be professional at all at the moment, so work away.
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To make a new page with all the right formatting, navigate to the page you want to create, click edit (you have to be logged in) and paste the following into the edit box, then work away. (I find [http://www.mediawiki.org/wiki/Help:Contents this] helpful.)
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Latest revision as of 01:20, 22 September 2011

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OVERVIEW
home

Abstract

We investigated the properties of a novel yet under-researched group of proteins called reflectins.

Reflectins are interesting and unusual because of their self-organising properties, which cause them to produce dynamic structural colour.

We expressed codon-optimised reflectin in E. coli, and investigated the proteins' in vivo and in vitro effects.

Structural Colour

Nature’s colours don’t just come from pigments, but from structure too.

Cephalopods camouflage themselves using intracellular structures made from reflectins.

These are the only known proteinaceous structures to exhibit structural colour. They are inspiring a new class of responsive optical materials.
Image 1
Image 2
Image 3

Impact of iGEM

We wanted to know what effect the iGEM competition has had on its past contestants.

We were also interested in what past participants thought of iGEM and how it shaped their view of synthetic biology.

We got in touch with a number of iGEM alumni and asked them to share their experience.

Tools

We used Gibson Assembly exclusively to build our constructs during the competition. This allowed us to do multi-part assemblies faster and with fewer bugs than if we had used standard techniques.

We contributed to a collection of software tools called Gibthon (initiated by Cambridge iGEM 2010), which aids the design of primers for Gibson assembly.

Retrieved from "http://2011.igem.org/Team:Cambridge"