Team:Cambridge

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Latest revision as of 01:20, 22 September 2011

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Abstract

We investigated the properties of a novel yet under-researched group of proteins called reflectins.

Reflectins are interesting and unusual because of their self-organising properties, which cause them to produce dynamic structural colour.

We expressed codon-optimised reflectin in E. coli, and investigated the proteins' in vivo and in vitro effects.

Structural Colour

Nature’s colours don’t just come from pigments, but from structure too.

Cephalopods camouflage themselves using intracellular structures made from reflectins.

These are the only known proteinaceous structures to exhibit structural colour. They are inspiring a new class of responsive optical materials.

Impact of iGEM

We wanted to know what effect the iGEM competition has had on its past contestants.

We were also interested in what past participants thought of iGEM and how it shaped their view of synthetic biology.

We got in touch with a number of iGEM alumni and asked them to share their experience.

Tools

We used Gibson Assembly exclusively to build our constructs during the competition. This allowed us to do multi-part assemblies faster and with fewer bugs than if we had used standard techniques.

We contributed to a collection of software tools called Gibthon (initiated by Cambridge iGEM 2010), which aids the design of primers for Gibson assembly.

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        <p>We investigated the properties of a novel yet under-researched group of proteins called reflectins.</p>
-       <p>Reflectins are interesting because of their self-organising properties, which cause them to produce dynamic structural colour.</p>
+       <p>Reflectins are interesting and unusual because of their self-organising properties, which cause them to produce dynamic structural colour.</p>
-       <p>We expressed codon-optimised reflectin in E.Coli, and observed the protein's in vivo and in vitro effects.</p>
+       <p>We expressed codon-optimised reflectin in E. coli, and investigated the proteins' in vivo and in vitro effects.</p>
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-         <p>We wanted to know whether the iGEM has had any effect on its previous contestants.</p>
+         <p>We wanted to know what effect the iGEM competition has had on its past contestants.</p>
-         <p>To this end, we conducted a number of interviews with previous iGEMmers, as well as conducting a simple poll of past contestants.</p>
+         <p>We were also interested in what past participants thought of iGEM and how it shaped their view of synthetic biology.</p>
+        <p>We got in touch with a number of iGEM alumni and asked them to share their experience.</p>
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-         <p>We used Gibson Assembly instead exclusively for construct assembly during the competition, allowing us to assemble plasmids faster and with greater ease than would be possible using standard techniques.</p>
+         <p>We used Gibson Assembly exclusively to build our constructs during the competition. This allowed us to do multi-part assemblies faster and with fewer bugs than if we had used standard techniques.</p>
          <p>We contributed to a collection of software tools called <i>Gibthon</i> (initiated by Cambridge iGEM 2010), which aids the design of primers for Gibson assembly.</p>
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