Team:Cambridge/Experiments/Assembly of Reflectin Constructs

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(Created page with "{{Template:Team:Cambridge/CAM_2011_TEMPLATE_HEAD}} This is a placeholder. We should fill it in. ==Construct Design== ==Primer Design== ==Assembly: first attempt== ===PCR=== ===...")
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==Assembly: first attempt==
==Assembly: first attempt==
===PCR===
===PCR===
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In the first round of PCR, we amplified fragments required for the assembly of GA1, GA2, GA3 and GA4 constructs.
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===Gibson Assembly===
===Gibson Assembly===
===Transformation===
===Transformation===

Revision as of 11:16, 19 August 2011

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OVERVIEW
home

This is a placeholder. We should fill it in.

Contents

Construct Design

Primer Design

Assembly: first attempt

PCR

In the first round of PCR, we amplified fragments required for the assembly of GA1, GA2, GA3 and GA4 constructs.

Gibson Assembly

Transformation

Results

Diagnostics

Assembly: second attempt

PCR

Gibson Assembly

Transformation

Results

What next?