Team:Brown-Stanford/PowerCell/Methods

From 2011.igem.org

(Difference between revisions)

Revision as of 23:29, 17 August 2011

REGObricks
Intro --------------------- ISRU --------------------- Biocement --------------------- S. pasteurii --------------------- Balloon
Flights --------------------- Transforming --------------------- Biobrick²
PowerCell
Intro --------------------- Cyanobacteria --------------------- Mars --------------------- Nutrients
FRETcetera
Intro --------------------- FRET etc. --------------------- Construct
Lab
Team --------------------- Parts --------------------- Notebook --------------------- Protocols --------------------- Safety
Partners
Collab --------------------- Sponsors
Outreach
SB5.0 --------------------- NASA --------------------- Lunar Science --------------------- Maker Faire --------------------- BBC --------------------- CBricks --------------------- Ethics --------------------- Regional
Jamboree

Methods and Materials

Cyanobacterial cultures: we used wild type strains of Anabaena PCC7120, Synechocystis PCC6803, Nostoc punctiforme ATCC 29133, and Synechococcus elongatus PCC 7942, all courtesy of James Golden. These were raised at 30˚C with shaking at 121rpm and ~40µEinsteins of light in liquid BG11 media.

Brown-Stanford SucroseSecretionDevice.jpg

Rippka, R., J. Deruelles, J. B Waterbury, M. Herdman, and R. Y Stanier. 1979. “Generic assignments, strain histories and properties of pure cultures of cyanobacteria.” Journal of General Microbiology 111 (1): 1.

@@ Line 2: / Line 2: @@
 == '''Methods and Materials''' ==
-Before bringing synthetic organisms to do work on Mars, one needs to answer the question of what to feed the cultures. Bringing media and nutrients from the Earth to supply large-scale bioreactors is slow and inefficient. PowerCell is a cellular energy generator engineered with blue-green algae (cyanobacteria) to fix atmospheric N2 and CO2 and export them to other cells.  When grown alongside synthetic Escherichia coli, these highly compatible “power plant” cells will fuel the generation of useful products such as medicine, heat, food, and other necessities. Our project aims to prove that cyanobacteria co-cultured with E. coli can become a self-sustaining system.
-[[File:Brown-Stanford InvA.png|center|400px]]
+'''Cyanobacterial cultures''': we used wild type strains of Anabaena PCC7120, Synechocystis PCC6803, Nostoc punctiforme ATCC 29133, and Synechococcus elongatus PCC 7942, all courtesy of James Golden.  These were raised at 30˚C with shaking at 121rpm and ~40µEinsteins of light in liquid BG11 media.
 [[File:Brown-Stanford SucroseSecretionDevice.jpg|center|700px]]
 {{:Team:Brown-Stanford/Templates/Foot}}
+Rippka, R., J. Deruelles, J. B Waterbury, M. Herdman, and R. Y Stanier. 1979. “Generic assignments, strain histories and properties of pure cultures of cyanobacteria.” Journal of General Microbiology 111 (1): 1.