Team:Brown-Stanford/PowerCell/Methods

From 2011.igem.org

REGObricks
Intro --------------------- ISRU --------------------- Biocement --------------------- S. pasteurii --------------------- Balloon
Flights --------------------- Transforming --------------------- Biobrick²
PowerCell
Intro --------------------- Cyanobacteria --------------------- Mars --------------------- Nutrients
FRETcetera
Intro --------------------- FRET etc. --------------------- Construct
Lab
Team --------------------- Parts --------------------- Notebook --------------------- Protocols --------------------- Safety
Partners
Collab --------------------- Sponsors
Outreach
SB5.0 --------------------- NASA --------------------- Lunar Science --------------------- Maker Faire --------------------- BBC --------------------- CBricks --------------------- Ethics --------------------- Regional
Jamboree

Methods and Materials

Cyanobacterial cultures: we used wild type strains of Anabaena PCC7120, Synechocystis PCC6803, Nostoc punctiforme ATCC 29133, and Synechococcus elongatus PCC 7942, all courtesy of James Golden. These were raised at 30˚C with shaking at 121rpm and ~40µEinsteins of light in liquid BG11 media¹.

Brown-Stanford AnabaenaVegetativeCellSecretionDevice.jpg

Brown-Stanford AnabaenaVegetativeCellReporterDevice.jpg

Brown-Stanford AnabaenaVegetativeCellSecretionReporterDevice.jpg

¹ Rippka, R., J. Deruelles, J. B Waterbury, M. Herdman, and R. Y Stanier. 1979. “Generic assignments, strain histories and properties of pure cultures of cyanobacteria.” Journal of General Microbiology 111 (1): 1.