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Team:Cambridge/Protocols/Extraction of cleaner genomic DNA from squid
From 2011.igem.org
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==Practice== | ==Practice== | ||
| - | 1. | + | 1. Prepare squid tissue samples and at least 100μl of DNA extraction buffer per tissue sample. |
| - | *''''' | + | *'''''DNA Extraction Buffer''''' |
| - | :# | + | :# 10mM Tris pH 8.2 |
| - | :# | + | :# 10mM EDTA |
| + | :# 200 mM NaCl | ||
| + | :# 0.5% SDS | ||
| + | :# 200 g/ml | ||
:#* '''<additional notes/important information regarding the previous step>''' | :#* '''<additional notes/important information regarding the previous step>''' | ||
Revision as of 12:34, 1 August 2011
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Extraction of cleaner genomic DNA from squid
As the quick and dirty method to extract genomic DNA failed to produce genomic DNA suitable for PCR, two new protocols are carried out as an attempt to obtain cleaner genomic DNA. This protocol produces clearner preparations and probably somewhat higher yields of genomic DNA.
Theory
How it works
Practice
1. Prepare squid tissue samples and at least 100μl of DNA extraction buffer per tissue sample.
- DNA Extraction Buffer
- 10mM Tris pH 8.2
- 10mM EDTA
- 200 mM NaCl
- 0.5% SDS
- 200 g/ml
- <additional notes/important information regarding the previous step>
the text within the < > is what should be written, don't include < > in actual writeup :P
if in doubt see the gel electrophoresis protocol
}
Safety
The safety implication of the procedure.
