Team:Cambridge/Labwork/Protocols
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A list of all protocols developed during the project. To add one, use the section below. | A list of all protocols developed during the project. To add one, use the section below. | ||
- | + | '''Amplification of DNA''' | |
- | + | ||
- | + | *[[Team:Cambridge/Protocols/PCR | Polymerase Chain Reaction]] : A method for amplifying a section of DNA | |
- | + | ||
- | + | '''Analysis of DNA''' | |
- | + | ||
- | + | *[[Team:Cambridge/Protocols/Gel_Electrophoresis |Gel Electrophoresis]] : A method used to separate DNA fragments of different sizes | |
- | + | *[[Team:Cambridge/Protocols/Gel Extraction of DNA |Gel Extraction of DNA]] : A technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis | |
- | + | *[[Team:Cambridge/Protocols/DNA Precipitation |Rescue Precipitation of DNA]] : Creating a clean DNA solution after dissolving agarose gel. | |
- | + | *[[Team:Cambridge/Protocols/Restriction_Enzyme_Digestion | Restriction Enzyme Digestion]] : A method for creating a restriction map of a plasmid | |
- | + | ||
- | + | '''Preparation of DNA Constructs''' | |
+ | |||
+ | *[[Team:Cambridge/Protocols/Gibson_Assembly |Gibson Assembly]] : A relatively new technique of joining multiple DNA strands reliably | ||
+ | |||
+ | '''Transformation of Bacterial Cells''' | ||
+ | |||
+ | *[[Team:Cambridge/Protocols/Making Competent Cells | Making Competent Cells]] : The methods required to make various cells competent | ||
+ | *[[Team:Cambridge/Protocols/Transformation_of_E.Coli |Transformation of E.Coli]] : A simple method of transforming competent E.coli with your DNA of choice | ||
+ | *[[Team:Cambridge/Protocols/Transformation_of_B._subtilis | Tranformation of B. subtilis]] : A technique used to introduce foreign DNA into Bacillus cells. | ||
+ | |||
+ | '''Bacterial Cultures''' | ||
+ | |||
+ | *[[Team:Cambridge/Protocols/Overnight_Culture |E. coli cell culture]] : A method for growing a cell culture in liquid medium | ||
+ | |||
+ | '''Extraction of DNA from Cells" | ||
+ | |||
+ | *[[Team:Cambridge/Protocols/Mini_Prep |Mini Prep]] : Extracting DNA from Bacterial Cells | ||
+ | *[[Team:Cambridge/Protocols/Extraction_of_genomic_DNA_from_squid | Extraction of genomic DNA from squid]] : A method to extract genomic DNA from squid tissue | ||
==Adding new Protocols== | ==Adding new Protocols== |
Revision as of 09:58, 20 July 2011
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Protocols
A list of all protocols developed during the project. To add one, use the section below.
Amplification of DNA
- Polymerase Chain Reaction : A method for amplifying a section of DNA
Analysis of DNA
- Gel Electrophoresis : A method used to separate DNA fragments of different sizes
- Gel Extraction of DNA : A technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis
- Rescue Precipitation of DNA : Creating a clean DNA solution after dissolving agarose gel.
- Restriction Enzyme Digestion : A method for creating a restriction map of a plasmid
Preparation of DNA Constructs
- Gibson Assembly : A relatively new technique of joining multiple DNA strands reliably
Transformation of Bacterial Cells
- Making Competent Cells : The methods required to make various cells competent
- Transformation of E.Coli : A simple method of transforming competent E.coli with your DNA of choice
- Tranformation of B. subtilis : A technique used to introduce foreign DNA into Bacillus cells.
Bacterial Cultures
- E. coli cell culture : A method for growing a cell culture in liquid medium
Extraction of DNA from Cells"
- Mini Prep : Extracting DNA from Bacterial Cells
- Extraction of genomic DNA from squid : A method to extract genomic DNA from squid tissue
Adding new Protocols
To add a new protocol, enter the name in the box below and click new to create the new page. You must then return to this page in order to add in a link to the page by copying the code below.
Add a new link on this page with
#[[Team:Cambridge/Protocols/PROTOCOL_NAME_HERE |PROTOCOL NAME HERE]] : Insert description here