Team:Cambridge/Labwork/Protocols

From 2011.igem.org

(Difference between revisions)
(Protocols)
(Protocols)
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A list of all protocols developed during the project. To add one, use the section below.
A list of all protocols developed during the project. To add one, use the section below.
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#[[Team:Cambridge/Protocols/PCR | Polymerase Chain Reaction]] : A method for amplifying a section of DNA
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'''Amplification of DNA'''
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#[[Team:Cambridge/Protocols/Gel_Electrophoresis |Gel Electrophoresis]] : A method used to separate DNA fragments of different sizes
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#[[Team:Cambridge/Protocols/Gel Extraction of DNA |Gel Extraction of DNA]] :  A technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis
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*[[Team:Cambridge/Protocols/PCR | Polymerase Chain Reaction]] : A method for amplifying a section of DNA
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#[[Team:Cambridge/Protocols/DNA Precipitation |Rescue Precipitation of DNA]] : Creating a clean DNA solution after dissolving agarose gel.
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#[[Team:Cambridge/Protocols/Gibson_Assembly |Gibson Assembly]] : A relatively new technique of joining multiple DNA strands reliably
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'''Analysis of DNA'''
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#[[Team:Cambridge/Protocols/Transformation_of_E.Coli |Transformation of E.Coli]] : A simple method of transforming competent E.coli with your DNA of choice
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#[[Team:Cambridge/Protocols/Transformation_of_B._subtilis | Tranformation of B. subtilis]] : A technique used to introduce foreign DNA into Bacillus cells.  
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*[[Team:Cambridge/Protocols/Gel_Electrophoresis |Gel Electrophoresis]] : A method used to separate DNA fragments of different sizes
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#[[Team:Cambridge/Protocols/Mini_Prep |Mini Prep]] : Extracting DNA from Bacterial Cells
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*[[Team:Cambridge/Protocols/Gel Extraction of DNA |Gel Extraction of DNA]] :  A technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis
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#[[Team:Cambridge/Protocols/Overnight_Culture |E. coli cell culture]] : A method for growing a cell culture in liquid medium
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*[[Team:Cambridge/Protocols/DNA Precipitation |Rescue Precipitation of DNA]] : Creating a clean DNA solution after dissolving agarose gel.
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#[[Team:Cambridge/Protocols/Restriction_Enzyme_Digestion | Restriction Enzyme Digestion]] : A method for creating a restriction map of a plasmid
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*[[Team:Cambridge/Protocols/Restriction_Enzyme_Digestion | Restriction Enzyme Digestion]] : A method for creating a restriction map of a plasmid
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#[[Team:Cambridge/Protocols/Extraction_of_genomic_DNA_from_squid | Extraction of genomic DNA from squid]] : A method to extract genomic DNA from squid tissue
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#[[Team:Cambridge/Protocols/Making Competent Cells | Making Competent Cells]] : The methods required to make various cells competent
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'''Preparation of DNA Constructs'''
 +
 
 +
*[[Team:Cambridge/Protocols/Gibson_Assembly |Gibson Assembly]] : A relatively new technique of joining multiple DNA strands reliably
 +
 
 +
'''Transformation of Bacterial Cells'''
 +
 
 +
*[[Team:Cambridge/Protocols/Making Competent Cells | Making Competent Cells]] : The methods required to make various cells competent
 +
*[[Team:Cambridge/Protocols/Transformation_of_E.Coli |Transformation of E.Coli]] : A simple method of transforming competent E.coli with your DNA of choice
 +
*[[Team:Cambridge/Protocols/Transformation_of_B._subtilis | Tranformation of B. subtilis]] : A technique used to introduce foreign DNA into Bacillus cells.  
 +
 
 +
'''Bacterial Cultures'''
 +
 
 +
*[[Team:Cambridge/Protocols/Overnight_Culture |E. coli cell culture]] : A method for growing a cell culture in liquid medium
 +
 
 +
'''Extraction of DNA from Cells"
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 +
*[[Team:Cambridge/Protocols/Mini_Prep |Mini Prep]] : Extracting DNA from Bacterial Cells
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*[[Team:Cambridge/Protocols/Extraction_of_genomic_DNA_from_squid | Extraction of genomic DNA from squid]] : A method to extract genomic DNA from squid tissue
==Adding new Protocols==
==Adding new Protocols==

Revision as of 09:58, 20 July 2011

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OVERVIEW
home

Protocols

A list of all protocols developed during the project. To add one, use the section below.

Amplification of DNA

Analysis of DNA

Preparation of DNA Constructs

  • Gibson Assembly : A relatively new technique of joining multiple DNA strands reliably

Transformation of Bacterial Cells

Bacterial Cultures

Extraction of DNA from Cells"

Adding new Protocols

To add a new protocol, enter the name in the box below and click new to create the new page. You must then return to this page in order to add in a link to the page by copying the code below.

Add a new link on this page with

#[[Team:Cambridge/Protocols/PROTOCOL_NAME_HERE |PROTOCOL NAME HERE]] : Insert description here