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Team:Cambridge/Protocols/Restriction Enzyme Digestion
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* '''Master mix preparation''' | * '''Master mix preparation''' | ||
: 2.0μl of DNA | : 2.0μl of DNA | ||
| - | : 0.5μl of each restriction enzyme (or | + | : 0.5μl of each restriction enzyme (or the respective amount of water for the control with uncut plasmids) |
| - | : 0.1μl of BSA - acetylated Bovine Serum Albumin enhances the performance of restriction enzymes | + | : 0.1μl of BSA - ''acetylated Bovine Serum Albumin enhances the performance of restriction enzymes'' |
: 5.9μl of water | : 5.9μl of water | ||
: 1.0μl of 2×NEBuffer | : 1.0μl of 2×NEBuffer | ||
| + | |||
| + | * '''Check for the compatibility of restriction enzymes chosen''' | ||
| + | To check if the two selected restriction enzymes can perform effective catalysis in the same solution, go to the website of [http://www.neb.com New England Biolabs] → select | ||
===Safety=== | ===Safety=== | ||
Revision as of 13:52, 15 July 2011
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Restriction Enzyme Digestion
protocol description
Theory
How it works
Practice
- Master mix preparation
- 2.0μl of DNA
- 0.5μl of each restriction enzyme (or the respective amount of water for the control with uncut plasmids)
- 0.1μl of BSA - acetylated Bovine Serum Albumin enhances the performance of restriction enzymes
- 5.9μl of water
- 1.0μl of 2×NEBuffer
- Check for the compatibility of restriction enzymes chosen
To check if the two selected restriction enzymes can perform effective catalysis in the same solution, go to the website of [http://www.neb.com New England Biolabs] → select
Safety
The safety implication of the procedure.
