Team:Brown-Stanford/Lab/Protocols/CaCl

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== '''CaCl<sub>2</sub> Competency''' ==
== '''CaCl<sub>2</sub> Competency''' ==

Revision as of 18:39, 5 July 2011

Brown-Stanford
iGEM

CaCl2 Competency

Buffers

TfBI

  • 30mM KOAc
  • 50mM MnCl2-4H2O
  • 100mM KCl
  • 10mM CaCl2-2H2O
  • 15% Glycerol

TfBII

  • 10mM Na-MOPS pH 7
  • 75mM CaCl2
  • 10mM KCl
  • 10% Glycerol