"
Team:Brown-Stanford/Lab/Protocols/CaCl
From 2011.igem.org
(Difference between revisions)
| (2 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
{{:Team:Brown-Stanford/Templates/Protocol}} | {{:Team:Brown-Stanford/Templates/Protocol}} | ||
| + | <html><h1>Protocol Code: CT3</h1></html> | ||
== '''CaCl<sub>2</sub> Competency''' == | == '''CaCl<sub>2</sub> Competency''' == | ||
| Line 17: | Line 18: | ||
*10mM KCl | *10mM KCl | ||
*10% Glycerol | *10% Glycerol | ||
| - | |||
{{:Team:Brown-Stanford/Templates/Foot}} | {{:Team:Brown-Stanford/Templates/Foot}} | ||
Latest revision as of 18:18, 28 September 2011
-
REGObricks
Intro--------------------- ISRU--------------------- Biocement--------------------- S. pasteurii--------------------- Balloon
Flights--------------------- Transforming--------------------- Biobrick2 -
PowerCell
Intro--------------------- Cyanobacteria--------------------- Mars--------------------- Nutrients -
FRETcetera
-
Lab
-
Partners
-
Outreach
SB5.0--------------------- NASA--------------------- Lunar Science--------------------- Maker Faire--------------------- BBC--------------------- CBricks--------------------- Ethics--------------------- Regional
Jamboree
Protocol Code: CT3
CaCl2 Competency
Buffers
TfBI
- 30mM KOAc
- 50mM MnCl2-4H2O
- 100mM KCl
- 10mM CaCl2-2H2O
- 15% Glycerol
TfBII
- 10mM Na-MOPS pH 7
- 75mM CaCl2
- 10mM KCl
- 10% Glycerol
