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Team:Brown-Stanford/Lab/Protocols/ATCC1376
From 2011.igem.org
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| - | {{:Team:Brown-Stanford/Templates/ | + | {{:Team:Brown-Stanford/Templates/Protocol}} |
| + | <html><h1>Protocol Code: M3</h1></html> | ||
== '''ATCC medium: 1376 (''Bacillus pasteurii'' NH<sub>4</sub>-YE medium)''' == | == '''ATCC medium: 1376 (''Bacillus pasteurii'' NH<sub>4</sub>-YE medium)''' == | ||
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*20.0 g Yeast extract | *20.0 g Yeast extract | ||
| - | *10.0 g (NH<sub>4</sub>)2SO<sub>4</sub> | + | *10.0 g (75.7 mM) (NH<sub>4</sub>)2SO<sub>4</sub> |
*1.0 L 0.13 M Tris buffer (pH 9.0) | *1.0 L 0.13 M Tris buffer (pH 9.0) | ||
*20.0 g Agar (if needed) | *20.0 g Agar (if needed) | ||
Autoclave ingredients separately. No growth occurs when ingredients are sterilized together. | Autoclave ingredients separately. No growth occurs when ingredients are sterilized together. | ||
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{{:Team:Brown-Stanford/Templates/Foot}} | {{:Team:Brown-Stanford/Templates/Foot}} | ||
Latest revision as of 18:17, 28 September 2011
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Protocol Code: M3
ATCC medium: 1376 (Bacillus pasteurii NH4-YE medium)
- 20.0 g Yeast extract
- 10.0 g (75.7 mM) (NH4)2SO4
- 1.0 L 0.13 M Tris buffer (pH 9.0)
- 20.0 g Agar (if needed)
Autoclave ingredients separately. No growth occurs when ingredients are sterilized together.
