Team:Baltimore/Notebook

From 2011.igem.org

(Difference between revisions)
(→Notebook)
(→September)
 
(38 intermediate revisions not shown)
Line 1: Line 1:
-
 
<!--- The Mission, Experiments --->
<!--- The Mission, Experiments --->
 +
{{MajorPageBmore}}
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
==Notebook==  
-
!align="center"|[[Team:Baltimore|Home]]
+
For more information about the project click [[Dr. Tom's Notes]]
-
!align="center"|[[Team:Baltimore/Team|Team]]
+
-
!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Baltimore Official Team Profile]
+
-
!align="center"|[[Team:Baltimore/Project|Project]]
+
-
!align="center"|[[Team:Baltimore/Parts|Parts Submitted to the Registry]]
+
-
!align="center"|[[Team:Baltimore/Modeling|Modeling]]
+
-
!align="center"|[[Team:Baltimore/Notebook|Notebook]]
+
-
!align="center"|[[Team:Baltimore/Safety|Safety]]
+
-
!align="center"|[[Team:Baltimore/Attributions|Attributions]]
+
-
|}
+
-
 
+
-
 
+
-
==Notebook==
+
Run through for abstract and scheduling:
Run through for abstract and scheduling:
-
Start with plasmid with taq gene and pst1 gene
+
Start with plasmid with taq gene and pst1 site
Remove pst1 site from taq coding sequence
Remove pst1 site from taq coding sequence
Line 26: Line 14:
* PCR   
* PCR   
* Digestion
* Digestion
-
* Transformation
+
* [[Team:Baltimore/Notebook/Transformation | Transformation]]
* Screening (1day)
* Screening (1day)
** Dilute DNA
** Dilute DNA
Line 41: Line 29:
* Ligation with vector (could be vector with the terminator sequence, promoter and RBS)  
* Ligation with vector (could be vector with the terminator sequence, promoter and RBS)  
* Transformation
* Transformation
-
Add a promoter, transcriptional terminator, ribosome binding site (RBS)
+
[[Team:Baltimore/Notebook/bb_assemblies| Add a promoter, transcriptional terminator, ribosome binding site (RBS)]]
* Screen colonies (1 day)
* Screen colonies (1 day)
** Colony PCR
** Colony PCR
Line 51: Line 39:
Compare it to other enzymes and make sure it works
Compare it to other enzymes and make sure it works
 +
===Calendar===
 +
 +
----
 +
 +
====July====
 +
'''Week 1:'''  [[Thursday, July 7]];
 +
 +
'''Week 2:'''  [[Tuesday, July 12]];  [[Wednesday, July 13]];  [[Thursday, July 14]];  [[Friday, July 15]];  [[Saturday, July 16]];  [[Sunday, July 17]];
 +
 +
'''Week 3:'''  [[Tuesday, July 19]];  [[Wednesday, July 20]];  [[Thursday, July 21]];  [[Friday, July 22]];  [[Saturday, July 23]];  [[Sunday, July 24]];
 +
 +
'''Week 4:'''  [[Tuesday, July 26]];  [[Wednesday, July 27]];  [[Thursday, July 28]];  [[Friday, July 29]];  [[Saturday, July 30]];  [[Sunday, July 31]];
 +
 +
====August====
 +
'''Week 1:'''    [[Tuesday, August 2]];  [[Wednesday, August 3]];  [[Thursday, August 4]];  [[Friday, August 5]];  [[Saturday, August 6]];  [[Sunday, August 7]];
 +
 +
'''Week 2:'''    Week Off
 +
 +
'''Week 3:'''    [[Tuesday, August 16]];  [[Wednesday, August 17]];  [[Thursday, August 18]];  [[Friday, August 19]];  [[Saturday, August 20]];  [[Sunday, August 21]];
 +
 +
Beginning in Week 4, we will be tightening up our iGEM ship for a multitude of reasons. READ the [[New Rules]].
 +
 +
'''Week 4:'''    [[Tuesday, August 23]];  [[Wednesday, August 24]];  [[Thursday, August 25]];  [[Friday, August 26]];  [[Saturday, August 27]];  [[Sunday, August 28]];
 +
 +
'''Week 5:'''    [[Tuesday, August 30]];  [[Wednesday, August 31]];
 +
 +
====September====
 +
 +
'''Week 1:'''    [[Thursday, September 1]];  [[Friday, September 2]];  [[Saturday, September 3]];  [[Sunday, September 4]];
 +
 +
'''Week 2:''' [[Tuesday, September 6]]; [[Thursday, September 8]]; [[Friday, September 9]];  [[Saturday, September 10]];  [[Sunday, September 11]];
 +
 +
'''Week 3:''' [[Tuesday, September 13]]; [[Thursday, September 15]]; [[Friday, September 16]];  [[Saturday, September 17]];  [[Sunday, September 18]];
-
7-12-11
+
'''Week 4:''' [[Tuesday, September 20]]; [[Thursday, September 22]]; [[Friday, September 23]];  [[Saturday, September 24]];  [[Sunday, September 25]];
-
Made one agarose gel 1%
+
'''Week 5:''' [[Tuesday, September 27]]; [[Wednesday, September 28]]; [[ Thursday, September 29 - DEADLINE FOR EVERYTHING]];
-
--[[User:Mduley|Mduley]] 19:19, 12 July 2011 (CDT)
+

Latest revision as of 17:47, 25 September 2011

Home Team Project Parts Submitted to the Registry Modeling Notebook Solutions Safety Attributions Hardware Material Safety Data Sheets [http://partsregistry.org/assembly/libraries.cgi?id=35|MIT Parts Registry]

Contents

Notebook

For more information about the project click Dr. Tom's Notes

Run through for abstract and scheduling:

Start with plasmid with taq gene and pst1 site

Remove pst1 site from taq coding sequence

  • In order to remove the restriction site, do site directed mutagenesis (1day)
  • PCR
  • Digestion
  • Transformation
  • Screening (1day)
    • Dilute DNA
    • Colony PCR individually
    • Digestion of PCR product with pst1
    • Run gel ~2500bp

Add biobrick prefix/suffix to taq coding sequence

  • PCR and add the prefix and suffix as primers (one prefix has an extra AG-make sure to use the correct one) (1-2 days)
    • Run gel
    • Cut out of gel
  • Cut with restriction enzyme
  • Clean up DNA
  • Ligation with vector (could be vector with the terminator sequence, promoter and RBS)
  • Transformation

Add a promoter, transcriptional terminator, ribosome binding site (RBS)

  • Screen colonies (1 day)
    • Colony PCR
    • Restriction Digestion
    • Clean up DNA
  • Sequence (1 day)

Make taq protein

Compare it to other enzymes and make sure it works

Calendar

July

Week 1: Thursday, July 7;

Week 2: Tuesday, July 12; Wednesday, July 13; Thursday, July 14; Friday, July 15; Saturday, July 16; Sunday, July 17;

Week 3: Tuesday, July 19; Wednesday, July 20; Thursday, July 21; Friday, July 22; Saturday, July 23; Sunday, July 24;

Week 4: Tuesday, July 26; Wednesday, July 27; Thursday, July 28; Friday, July 29; Saturday, July 30; Sunday, July 31;

August

Week 1: Tuesday, August 2; Wednesday, August 3; Thursday, August 4; Friday, August 5; Saturday, August 6; Sunday, August 7;

Week 2: Week Off

Week 3: Tuesday, August 16; Wednesday, August 17; Thursday, August 18; Friday, August 19; Saturday, August 20; Sunday, August 21;

Beginning in Week 4, we will be tightening up our iGEM ship for a multitude of reasons. READ the New Rules.

Week 4: Tuesday, August 23; Wednesday, August 24; Thursday, August 25; Friday, August 26; Saturday, August 27; Sunday, August 28;

Week 5: Tuesday, August 30; Wednesday, August 31;

September

Week 1: Thursday, September 1; Friday, September 2; Saturday, September 3; Sunday, September 4;

Week 2: Tuesday, September 6; Thursday, September 8; Friday, September 9; Saturday, September 10; Sunday, September 11;

Week 3: Tuesday, September 13; Thursday, September 15; Friday, September 16; Saturday, September 17; Sunday, September 18;

Week 4: Tuesday, September 20; Thursday, September 22; Friday, September 23; Saturday, September 24; Sunday, September 25;

Week 5: Tuesday, September 27; Wednesday, September 28; Thursday, September 29 - DEADLINE FOR EVERYTHING;

Retrieved from "http://2011.igem.org/Team:Baltimore/Notebook"