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From 2011.igem.org

Tube Prep

Each tube contained 8 mL of LB broth and 8 µL of concentrated amphicillin 1000x. In addition, each tube contained a single colony. Each chosen colony was not near any other colonies to preserve diversity.

These tubes were from the Promoter Plate

• P1
• P2
• P3
• P4

These tubes were from the Terminator Plate

• T1
• T2
• T3
• T4

These tubes were from the Ribosome Binding Site Plate

• R1
• R2
• R3
• R4

Incubation

The tubes were placed in the incubator at 4:21 PM at 31 Celsius and 135 RPM. They should be removed in 24 hours, at 4:21 PM Friday.

Dilution of 7-14-11 PCR products from lanes 1B,2B,4B, 5A, and 5B

5.0 µl PCR reserved Product

12.5 µl 10x Buffer

107.5 µl Nuclease free water

125.0 µl Total

--TravisCR 20:27, 21 July 2011 (CDT) Team:Baltimore/Notebook

Hours

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