Thursday, July 21
Each tube contained 8 mL of LB broth and 8 µL of concentrated amphicillin 1000x. In addition, each tube contained a single colony. Each chosen colony was not near any other colonies to preserve diversity.
These tubes were from the Promoter Plate
These tubes were from the Terminator Plate
These tubes were from the Ribosome Binding Site Plate
The tubes were placed in the incubator at 4:21 PM at 31 Celsius and 135 RPM. They should be removed in 24 hours, at 4:21 PM Friday.
Dilution of 7-14-11 PCR products from lanes 1B,2B,4B, 5A, and 5B
- 5.0 µl PCR reserved Product
- 12.5 µl 10x Buffer
- 107.5 µl Nuclease free water
- 125.0 µl Total