Thursday, September 8




Travis- Can be there from 3:30-7:45.

  • Desirae: 7pm-9pm

We met today to discuss progress. The plasmid preps of the mutagenized pET-Taq plasmid (to check for the presence or absence of the PstI site) gave no yield of plasmid DNA. We looked at the remaining portion of the bacterial cultures from which the plasmids were prepared and decided that the culture might not have been dense enough. We decided to regrow the cultures until they were visibly cloudy before isolating the plasmid. We also discussed re-transforming the original mutagenesis products into competent bacteria using the iGEM protocol and spent some time preparing reagents for the transformation.