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Team:Baltimore/Notebook
From 2011.igem.org
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'''Week 3:''' [[Tuesday, September 13]]; [[Thursday, September 15]]; [[Friday, September 16]]; [[Saturday, September 17]]; [[Sunday, September 18]]; | '''Week 3:''' [[Tuesday, September 13]]; [[Thursday, September 15]]; [[Friday, September 16]]; [[Saturday, September 17]]; [[Sunday, September 18]]; | ||
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| + | '''Week 4:''' [[Tuesday, September 20]]; [[Thursday, September 22]]; [[Friday, September 23]]; [[Saturday, September 24]]; [[Sunday, September 25]]; | ||
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| + | '''Week 5:''' [[Tuesday, September 27]]; [[Wednesday, September 28]]; [[ Thursday, September 29 - DEADLINE FOR EVERYTHING]]; | ||
Latest revision as of 17:47, 25 September 2011
| Home | Team | Project | Parts Submitted to the Registry | Modeling | Notebook | Solutions | Safety | Attributions | Hardware | Material Safety Data Sheets | [http://partsregistry.org/assembly/libraries.cgi?id=35|MIT Parts Registry] |
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Contents |
Notebook
For more information about the project click Dr. Tom's Notes
Run through for abstract and scheduling:
Start with plasmid with taq gene and pst1 site
Remove pst1 site from taq coding sequence
- In order to remove the restriction site, do site directed mutagenesis (1day)
- PCR
- Digestion
- Transformation
- Screening (1day)
- Dilute DNA
- Colony PCR individually
- Digestion of PCR product with pst1
- Run gel ~2500bp
Add biobrick prefix/suffix to taq coding sequence
- PCR and add the prefix and suffix as primers (one prefix has an extra AG-make sure to use the correct one) (1-2 days)
- Run gel
- Cut out of gel
- Cut with restriction enzyme
- Clean up DNA
- Ligation with vector (could be vector with the terminator sequence, promoter and RBS)
- Transformation
Add a promoter, transcriptional terminator, ribosome binding site (RBS)
- Screen colonies (1 day)
- Colony PCR
- Restriction Digestion
- Clean up DNA
- Sequence (1 day)
Make taq protein
Compare it to other enzymes and make sure it works
Calendar
July
Week 1: Thursday, July 7;
Week 2: Tuesday, July 12; Wednesday, July 13; Thursday, July 14; Friday, July 15; Saturday, July 16; Sunday, July 17;
Week 3: Tuesday, July 19; Wednesday, July 20; Thursday, July 21; Friday, July 22; Saturday, July 23; Sunday, July 24;
Week 4: Tuesday, July 26; Wednesday, July 27; Thursday, July 28; Friday, July 29; Saturday, July 30; Sunday, July 31;
August
Week 1: Tuesday, August 2; Wednesday, August 3; Thursday, August 4; Friday, August 5; Saturday, August 6; Sunday, August 7;
Week 2: Week Off
Week 3: Tuesday, August 16; Wednesday, August 17; Thursday, August 18; Friday, August 19; Saturday, August 20; Sunday, August 21;
Beginning in Week 4, we will be tightening up our iGEM ship for a multitude of reasons. READ the New Rules.
Week 4: Tuesday, August 23; Wednesday, August 24; Thursday, August 25; Friday, August 26; Saturday, August 27; Sunday, August 28;
Week 5: Tuesday, August 30; Wednesday, August 31;
September
Week 1: Thursday, September 1; Friday, September 2; Saturday, September 3; Sunday, September 4;
Week 2: Tuesday, September 6; Thursday, September 8; Friday, September 9; Saturday, September 10; Sunday, September 11;
Week 3: Tuesday, September 13; Thursday, September 15; Friday, September 16; Saturday, September 17; Sunday, September 18;
Week 4: Tuesday, September 20; Thursday, September 22; Friday, September 23; Saturday, September 24; Sunday, September 25;
Week 5: Tuesday, September 27; Wednesday, September 28; Thursday, September 29 - DEADLINE FOR EVERYTHING;
