Team:Brown-Stanford/Lab/Protocols/ATCC1376
From 2011.igem.org
(Difference between revisions)
(→ATCC medium: 1376 Bacillus pasteurii NH4-YE medium) |
(→ATCC medium: 1376 Bacillus pasteurii NH4-YE medium) |
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{{:Team:Brown-Stanford/Templates/Main}} | {{:Team:Brown-Stanford/Templates/Main}} | ||
- | == '''ATCC medium: 1376 ''Bacillus pasteurii'' NH<sub>4</sub>-YE medium''' == | + | == '''ATCC medium: 1376 (''Bacillus pasteurii'' NH<sub>4</sub>-YE medium)''' == |
*20.0 g Yeast extract | *20.0 g Yeast extract | ||
*10.0 g (NH<sub>4</sub>)2SO<sub>4</sub> | *10.0 g (NH<sub>4</sub>)2SO<sub>4</sub> |
Revision as of 23:13, 11 June 2011
ATCC medium: 1376 (Bacillus pasteurii NH4-YE medium)
- 20.0 g Yeast extract
- 10.0 g (NH4)2SO4
- 1.0 L 0.13 M Tris buffer (pH 9.0)
- 20.0 g Agar (if needed)
Autoclave ingredients separately. No growth occurs when ingredients are sterilized together.