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Team:Brown-Stanford/Lab/Protocols/ATCC1376
From 2011.igem.org
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(→ATCC medium: 1376 Bacillus pasteurii NH4-YE medium) |
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{{:Team:Brown-Stanford/Templates/Main}} | {{:Team:Brown-Stanford/Templates/Main}} | ||
| - | == '''ATCC medium: 1376 ''Bacillus pasteurii'' | + | == '''ATCC medium: 1376 ''Bacillus pasteurii'' NH<sub>4</sub>-YE medium''' == |
| - | 20.0 g Yeast extract | + | *20.0 g Yeast extract |
| - | 10.0 g (NH<sub>4</sub>)2SO<sub>4</sub> | + | *10.0 g (NH<sub>4</sub>)2SO<sub>4</sub> |
| - | 1.0 L 0.13 M Tris buffer (pH 9.0) | + | *1.0 L 0.13 M Tris buffer (pH 9.0) |
| - | 20.0 g Agar (if needed) | + | *20.0 g Agar (if needed) |
Autoclave ingredients separately. No growth occurs when ingredients are sterilized together. | Autoclave ingredients separately. No growth occurs when ingredients are sterilized together. | ||
{{:Team:Brown-Stanford/Templates/Foot}} | {{:Team:Brown-Stanford/Templates/Foot}} | ||
Revision as of 23:13, 11 June 2011
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Jamboree
ATCC medium: 1376 Bacillus pasteurii NH4-YE medium
- 20.0 g Yeast extract
- 10.0 g (NH4)2SO4
- 1.0 L 0.13 M Tris buffer (pH 9.0)
- 20.0 g Agar (if needed)
Autoclave ingredients separately. No growth occurs when ingredients are sterilized together.
