Team:Amsterdam/29 August 2011

From 2011.igem.org

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29-08-2011

Growth curves

Measuring growth curves off our wild type (TOP 10), at 4, 8, 15, and 21°C.

Construct check

The ON samples were minprepped, digested with EcorI and PstI; and checked on gel.

New constructs

The constructs were digested to add extra parts. The following constructs were made using the assembly protocol from Delft 2010:

  • R0010+ B0034 + CspC
  • R0010+ B0034 + Cpn10
  • R0010+ B0034 + Cpn60
  • R0010+ B0034 + Shednak
  • K206000+ B0034 + CspC
  • K206000+ B0034 + Cpn10
  • K206000+ B0034 + Cpn60
  • K206000+ B0034 + Shednak