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Uppsala-Sweden/7 July 2011
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| + | re-circularized of pigment vectors after site mutation PCR (Purification protocol, Phosphorylation of DNA, DpnI digestion protocol). Transformation of the ligated plasmids from 11-07-06 (Protocol for transforming TOP10 competent cells). Transformation of mutagenized amilCP and amilGFP. Digestion of ccaS, ccaR and plasmid backbone plasmid pSB1C3 (BioBrick Assembly Manual). | ||
Latest revision as of 20:25, 20 July 2011
Welcome to Uppsala-SwedeniEM '2011
Notebook
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2011-07-07
re-circularized of pigment vectors after site mutation PCR (Purification protocol, Phosphorylation of DNA, DpnI digestion protocol). Transformation of the ligated plasmids from 11-07-06 (Protocol for transforming TOP10 competent cells). Transformation of mutagenized amilCP and amilGFP. Digestion of ccaS, ccaR and plasmid backbone plasmid pSB1C3 (BioBrick Assembly Manual).
