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Uppsala-Sweden/13 July 2011
From 2011.igem.org
Welcome to Uppsala-SwedeniEM '2011
Notebook
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2011-07-13
We started the day by running EnvZ-KO, ccaS, EcoRI- digested amilCP and amilGFP on a gel. We obtained good result since there were no distinguishable multiple bands in the gel, although the “single band” seemed a bit smeared. Then we did transformation of ccaS_EcoRI, amilGFP_PstI, pSB1A3-amilCP, pSB-pcpcG2 and pSB4A5 backbone. Re-streak (There were one colony on the whole plate) and PCR of tetR inverter (DNA taken from well distributed from MIT).
o/n of B1001 terminator, PlacO, PSB3T5 and PSB4K5-backbone (prepared for frozen stock + plasmid preparation) and finally RFP with RBS and lambda c1.inv (prepared for frozen stock + plasmid preparation). The last thing we did was to make new plates of all the three antibiotics (Am,k, Ch). We also made some plate with Tetracyclin
