Uppsala-Sweden/11 July 2011

From 2011.igem.org







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2011-07-11

Today we did a frozen stock of all viable stocks in -80°C freezer (ccaS clone 1,RBS-pcyA, amilGFP, amilCP, ccaR, RBS-ho1, PompC-Inv.cI). We also did a 3ml culture of the two clones of ccaR, RBS-pcyA and RBS-ho1, the constructs needed for the upcoming assemblies. Furthermore, we did a plasmid preparation of this clones. Transformation of tetR and lambda CI from MIT distribution. PCR of amilCP to add BioBrick prefix and suffix. Mutagensis of amilGFP-PstI and ccaS mutagenesis, round one.