"
Uppsala-Sweden/12 July 2011
From 2011.igem.org
Welcome to Uppsala-SwedeniEM '2011
Notebook
|
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2011-07-12
The day started by running a gel of ccaS mutant, amilCP-BB10, and amilGFP-PstI. At the same time we prepared the sample (ccaR, ccaS, RBS-ho1 and RBS-pcyA) for sequencing. Plating of terminator B1001, PLlacO, pSB3T5 and pSB4K5. PCR purification of ccaS, amilGFP, amilCP, clone amilCP and PcpcG2 (Purification protocol). Re-ligation and transform mutagenized ccaS and amilGFP. Transformation of amilCP, clone amilCP, TetR inverter and pcpcG2. Re-streak of cI inverter transformants from 2011-07-11.
The transformation of inverters from yesterday didn’t go well. The lambda-cI-inverter had only a few colonies, while the tetR inverter had no colonies at all. So TetR inverter has to be redone. This time the transformation used 2 µl of DNA from the MIT distribution.
Furthermore new plates containing all antibiotics and some containing teracyclin were made.
