Uppsala-Sweden/29 June 2011

From 2011.igem.org







Notebook

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31

2011-06-29

Transformation of BioBrick plasmids (protocol for transforming TOP10 competent cells):

pSB2K3-Q03530 (cII inverter) pSB3T5-J04450 (low copy vector tetR, ori P15A) pSB1AK3-B0015 (Double terminator) pSB2K3-Q01511 (cI inverter) pSB1A2-R0082 (PompC) pSB1A2-K093005 (RBS + RFP, red dye) pSB2K3-I15010 (cph8 red sensor) - had to be redone due to failure in the first attempt.

Followed up by restreaking of the transformants from the previous day.