Uppsala-Sweden/4 July 2011

From 2011.igem.org







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2011-07-04

Started by doing overnight cultures of the strains carrying these plasmids:

pSB1A3-J04450 (vector, ampR) pSB1C3-J04450 (vector, CmR) pSB1K3-J04450 (vector, KanR) pSB1A2-B0034 (Standard RBS) pSB2K3-I15008 (ho1, chromophore gene) pSB2K3-I15009 (pcyA, chomophore) pSB2K3-Q03530 (cII inverter) pSB1AK3-B0015 (Double terminator) pSB2K3-Q01511 (cI inverter) pSB1A2-R0082 (PompC) pSB2K3-I15010 (cph8 red sensor)

The purpose was to make plasmid preparation the day after.