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Uppsala-Sweden/29 June 2011
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| + | 2011-06-29 | ||
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| + | Transformation of BioBrick plasmids (protocol for transforming TOP10 competent cells): | ||
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| + | pSB2K3-Q03530 (cII inverter) pSB3T5-J04450 (low copy vector tetR, ori P15A) pSB1AK3-B0015 (Double terminator) pSB2K3-Q01511 (cI inverter) pSB1A2-R0082 (PompC) pSB1A2-K093005 (RBS + RFP, red dye) pSB2K3-I15010 (cph8 red sensor) - had to be redone due to failure in the first attempt. | ||
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| + | Followed up by restreaking of the transformants from the previous day. | ||
Latest revision as of 20:21, 20 July 2011
Welcome to Uppsala-SwedeniEM '2011
Notebook
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2011-06-29
Transformation of BioBrick plasmids (protocol for transforming TOP10 competent cells):
pSB2K3-Q03530 (cII inverter) pSB3T5-J04450 (low copy vector tetR, ori P15A) pSB1AK3-B0015 (Double terminator) pSB2K3-Q01511 (cI inverter) pSB1A2-R0082 (PompC) pSB1A2-K093005 (RBS + RFP, red dye) pSB2K3-I15010 (cph8 red sensor) - had to be redone due to failure in the first attempt.
Followed up by restreaking of the transformants from the previous day.
