Team:Amsterdam/31 August 2011
From 2011.igem.org
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31-08-2011
Transformation check
Only the positive control showed colonies on the plate
- The construct making will be redone
Constructs
Construct were made according to assembly protocol of Delft 2010.
Digestion
Digestion of vectors with SpeI and PstI:
- R0010_pSB1A2
- K206000_pSBA3
- R0010_B0034_pSB1A2
Digestion of inserts with XbaI and PstI:
- Shednak
- CspC
- Cpn10
- Cpn60
- B0034_Cpn10_pSB1A2
- B0034_Cpn60_pSB1A2
- B0034_CspC_pSB1A2
Purification
The vectors were purified with size selective purification. The inserts were purified on agarose gel.
Ligation
The following constructs were ligated ON at 16 degrees Celcius:
- R0010+ B0034 + CspC
- R0010+ B0034 + Cpn10
- R0010+ B0034 + Cpn60
- R0010+ B0034 + Shednak
- K206000+ B0034 + CspC
- K206000+ B0034 + Cpn10
- K206000+ B0034 + Cpn60
- K206000+ B0034 + Shednak
Growth curves
Still measuring the growth curves for 4 and 8°C