Team:Amsterdam/30 August 2011
From 2011.igem.org
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30-08-2011 | Meeting 25
Notulen meeting 30 augustus
Present: Paul, Domenico, Freek, Lisette, Isoude, Bas, Sandra Absent: Jorick, Yuri, Pernette
Lab work
Assemblies look promising.
Parts from Yale are shipped and passed customs.
Growth curve experiments: Measurements of wild type are finished. Experiments with the new strains can start anytime. Minor problem: Not enough material. Domenico will ask if we can borrow equipment from the VU.
Bas made a model. Domenico and Lisette will have a look at it. Also Douwe should check it.
Human outreach
Presentation in Utrecht went really well. Wednesday: Jantine will give a presentation at the VU. And in the afternoon we have a meeting at Nemo, Sandra and Jantine will go. Thursday: Delft Day. New meeting of the Minds discussion day. Domenico, Jantine and Sandra will go.
Collaboration: Paul is testing the project of Wageningen University. Software program they send works. He is still working on the CFP test.
We like to remember our supervisor to his promise: Domenico has donated his body to our project and we get to shave his hair if we go to Boston.
Labwork
Purification of digests with "Size selective precipitation" or on agarose gel. Ligation and transformation of the following constructs into TOP10:
- R0010+ B0034 + CspC
- R0010+ B0034 + Cpn10
- R0010+ B0034 + Cpn60
- R0010+ B0034 + Shednak
- K206000+ B0034 + CspC
- K206000+ B0034 + Cpn10
- K206000+ B0034 + Cpn60
- K206000+ B0034 + Shednak
Growth curves
Still measuring the growth curves for 4 and 8°C