Team:Brown-Stanford/Lab/Protocols/SOB

From 2011.igem.org

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<html><h1>Protocol Code: M6</h1></html>
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== '''SOB''' ==
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== SOB ==
 
Composition:
Composition:
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*2.5 mL KCl (1M stock)
*2.5 mL KCl (1M stock)
*20 mL MgSO4 (1M stock)
*20 mL MgSO4 (1M stock)
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Latest revision as of 18:17, 28 September 2011

Brown-Stanford
iGEM

Protocol Code: M6

SOB

Composition:

  • 0.5% (w/v) yeast extract
  • 2% (w/v) tryptone
  • 10 mM NaCl
  • 2.5 mM KCl
  • 20 mM MgSO4

Per liter of SOB:

  • 5 g yeast extract
  • 20 g tryptone
  • 10 mL NaCl (1M stock)
  • 2.5 mL KCl (1M stock)
  • 20 mL MgSO4 (1M stock)