"
Team:Brown-Stanford/Lab/Protocols/Gibson
From 2011.igem.org
-
REGObricks
Intro--------------------- ISRU--------------------- Biocement--------------------- S. pasteurii--------------------- Balloon
Flights--------------------- Transforming--------------------- Biobrick2 -
PowerCell
Intro--------------------- Cyanobacteria--------------------- Mars--------------------- Nutrients -
FRETcetera
-
Lab
-
Partners
-
Outreach
SB5.0--------------------- NASA--------------------- Lunar Science--------------------- Maker Faire--------------------- BBC--------------------- CBricks--------------------- Ethics--------------------- Regional
Jamboree
Gibson Assembly
5x isothermal buffer
- 0.75g - 25% PEG-8000
- 1500 µL - 500 mM Tris-HCl pH 7.5
- 75 µL - 50mM MgCl2
- 150 µL - 50mM DTT
- 30 µL - 1mM dATP
- 30 µL - 1mM dTTP
- 30 µL - 1mM dCTP
- 30 µL - 1mM dGTP
- 300 µL - 5mM NAD
- Nuclease-free water to fill to 3000µL
Total - 3000 µL
